Extracting and purifying RNA from cultured cells is one of the most widely used applications. Traditionally this has been a highly repetitive and labor-intensive procedure requiring …
RNeasy Mini Kit — for purification of total RNA from animal cells, animal tissues, and yeast, and for cleanup of RNA from crude RNA preps and enzymatic reactions (e.g., DNase digestion, …
If growing in a monolayer, cells can be lysed directly in the plate or trypsinized and collected as a cell pellet prior to lysis. To lyse directly in cell culture plate: Determine approximate number of …
Sorted Cells. The issue with extracting RNA from sorted cells is the volume of cells after sorting must not dilute the denaturing agent beyond its ability to denature RNase. As such we …
Redissolving the RNA Remove supernatant. Air dry the pellet for 5-10 minutes. Do not completely dry out the pellet. Dissolve pellet in 30 to 60 μl RNase free water or 0.5% SDS by passing the …
The following compilation reflects the average yield of total RNA or mRNA from various sources and is intended to help the researcher to estimate the amount of tissue or cells required.
This protocol provides the instructions to extract RNA with good quality (A260/280 ratio close to 2.0 and yield more than 200 ng RNA per sample) RNA for qPCR experiment from cells …